| Strain, strain background (male Sprague-Dawley rats) |
WT Sprague-Dawley rats |
Biomedical Science Animal Facility of the University of Melbourne |
|
|
| Recombinant DNA reagent |
pAAV-hSyn-DIO-(hCAR)off-(ChETA-mRuby2)on-W3SL |
Addgene, gift from Prof. Adam Kepecs |
Addgene plasmid #111391 |
|
| Strain, strain background (AVV) |
AAV1/2-CAG-DIO-(hCAR)off- (GtACR2Kv2.1)on-W3SL |
In-house cloning and virus production |
|
2.04×1011 GC/ml |
| Strain, strain background (AVV) |
AAVDJ8-CBA-Cre-TdTomato-WPRE |
In-house cloning and virus production |
|
1.14×1012 GC/ml |
| Strain, strain background (AVV) |
AAVrg-EF1α-mCherry-IRES-cre |
Addgene |
Addgene # 55632
|
8×1012 GC/ml |
| Strain, strain background (AVV) |
AAVrg-CAG-GFP |
In-house cloning and virus production |
|
1.29×1013 GC/ml |
| Antibody |
Chicken polyclonal anti-GFP |
Aves Labs Inc |
Cat#: GFP-1010 |
1:5000 |
| Antibody |
Mouse monoclonal anti-TH |
Merck-Millipore |
Cat#: MAB318 |
1:5000 |
| Antibody |
Rabbit polyclonal anti-parvalbumin |
Abcam |
Cat#: ab11427 |
1:5000 |
| Antibody |
Goat polyclonal anti-ChAT |
Chemicon-Merck |
Cat#: AB144P |
1:1000 |
| Antibody |
Rabbit polyclonal anti-dsRed |
Takara Bio, Clontech |
Cat#: 632496 |
1:5000 |
| Antibody |
Goat polyclonal anti-mCherry |
Sicgen, Cantanhede |
Cat#: AB0040-200 |
1:5000 |
| Antibody |
Alexa488-conjugated donkey polyclonal anti-chicken |
Jackson ImmunoResearch Laboratories, Inc |
Cat#: 703545155 |
1:500 |
| Antibody |
Cy3-conjugated donkey polyclonal anti-rabbit |
Jackson ImmunoResearch Laboratories, Inc |
Cat#: 711165152 |
1:500 |
| Antibody |
Cy3-conjugated donkey polyclonal anti-goat |
Jackson ImmunoResearch Laboratories, Inc |
Cat#: 705165003 |
1:500 |
| Antibody |
Cy5-conjugated donkey polyclonal anti-mouse |
Jackson ImmunoResearch Laboratories, Inc |
Cat#: 715175151 |
1:500 |
| Antibody |
Cy5-conjugated donkey polyclonal anti-goat |
Jackson ImmunoResearch Laboratories, Inc |
Cat#: 705175147 |
1:500 |
| Antibody |
Biotin-SP-conjugated donkey polyclonal anti-goat |
Jackson ImmunoResearch Laboratories, Inc |
Cat#: 705065147 |
1:500 |
| Antibody |
Rabbit polyclonal anti-NK1R |
Sigma-Aldrich |
Cat # S8305 |
1:5000 |
| Antibody |
Streptavidin-Marina blue |
Invitrogen-Thermo Fisher Scientific |
Cat#: S11221 |
1:200 |
| Commercial assay or kit |
RNAscope multiplex fluorescence v1 kit |
ACD |
Cat #320851 |
|
| Commercial assay or kit |
Protease IV |
ACD |
Cat #322336 |
|
| Commercial assay or kit |
VGAT (SLC32A1) |
ACD |
Cat #ADV424541-C2 |
|
| Commercial assay or kit |
VGluT2 (Slc17a6) |
ACD |
Cat #317011-C3 |
|
| Commercial assay or kit |
SST |
ACD |
Cat#ADV412181-C3 |
|
| Commercial assay or kit |
Reelin |
ACD |
Cat ADV1048921- C1 |
|
| Commercial assay or kit |
C1 diluent |
ACD |
Cat #ADV300041 |
|
| Chemical compound, drug |
Meloxicam |
Lyppard |
|
1 mg/kg, s.c. |
| Chemical compound, drug |
Isoflurane |
Rhodia Australia |
|
5% induction, 2–2.5% maintenance |
| Chemical compound, drug |
Ketamine |
Lyppard |
|
75 mg/kg, i.p. |
| Chemical compound, drug |
Medetomidine |
Pfizer Animal Health |
|
0.5 mg/kg, i.p. |
| Chemical compound, drug |
Atipamazole |
Pfizer Animal Health |
|
1 mg/kg, i.p |
| Chemical compound, drug |
Urethane |
Sigma-Aldrich |
|
1.4 g/kg, i.v. |
| Chemical compound, drug |
Buprenorphine |
Schering-Plough |
|
0.025 mg/kg, s.c. |
| Software, algorithm |
Spike2 |
Cambridge Electrical Design |
|
|
| Software, algorithm |
Zen Blue |
Carl-Zeiss |
|
|
| Software, algorithm |
Zen Black |
Carl-Zeiss |
|
|
| Software, algorithm |
ImageJ |
NIH |
|
|
| Software, algorithm |
SigmaPlot v11 |
Systat Software Inc |
|
|
| Software, algorithm |
Prims v9.0 |
GraphPad |
|
|
