Fig 4.
CDPK1 and CDPK2A comprise a signaling module that regulates zaprinast-stimulated egress and the lytic cycle. (A) Partial inhibition of endogenous CDPK1G by 3-MB-PP1 (40 nM) leads to ablation of plaque formation by parasites conditionally depleted of CDPK2A. Scale bar is 400 µm. (B) Dose-response of CDPK1 inhibition with 3-MB-PP1 monitoring endpoint zaprinast-stimulated egress in parasites depleted of or expressing the indicated CDPK. Graphs are egress of IAA-treated parasites as percentage of the vehicle. Mean ± SEM plotted for n = 3 biological replicates. (C) EC50 (μM) for 3-MB-PP1 of CDPK-depleted or untreated parasites; significance was calculated by unpaired one-tailed t-test across n = 3 biological replicates.