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Relief of rTth inhibition due to blood by the addition of 5 μg of lima bean trypsin inhibitor in a PCR mixture containing 1 ng of L. monocytogenes DNA and blood at different concentrations. Lanes: 1, 100-bp molecular weight marker (Boehringer Mannheim); 2, negative control; 3, positive control; 4, 20% blood; 5, 2% blood; 6, 0.2% blood; 7, 0.04% blood; 8, 0.02% blood; 9, 0.004% blood.
