Table II.
Genotypic differences in influx, efflux, and unidirectional shoot uptake
Unidirectional transport across the plasma membrane and tonoplast of root cells, measured as 22Na+ uptake or efflux, and shoot uptake. Influx data represent slope of linear regressions estimated using least squares method and 95% confidence intervals (shoot uptake estimated between 1–8 h). Efflux data are presented as absolute rates and also as the proportion of the cytoplasmic (for plasma membrane) or vacuolar (for tonoplast) Na+ that was effluxed per minute (the relative rates).
Line 149 (Salt Tolerant) | Tamaroi (Salt Sensitive) | |
---|---|---|
Plasma membrane influx (nmol g FW−1 min−1) | 65.3 (46.8–83.9) | 67.4 (38.8–96.1) |
Tonoplast influx (nmol g FW−1 min−1) | ||
15 to 450 min | 11.0 (9.9–12.0) | 8.2 (6.9–9.5) |
0.3 to 24 h (dark subtracted) | 13.2 (12.1–14.2) | 12.4 (10.0–14.8) |
Shoot uptake (nmol g root FW−1 min−1) | 0.6 (−0.1–1.4) | 4.6 (3.2–6.0) |
Root efflux (nmol g FW−1 min−1) | ||
Plasma membrane | 335 | 108 |
Tonoplast | 5.2 | 3.5 |
Relative efflux (min−1) | ||
Plasma membrane efflux (min−1) | 0.05907 | 0.07261 |
Tonoplast efflux (min−1) | 0.00122 | 0.00071 |