TABLE 2.
List of missense variants in important drug resistance genes and targets a
| Gene name | Gene ID | Description | Genomic location | AA substitution (#variants/total callable) |
|---|---|---|---|---|
| DHFR-TS | PF3D7_0417200 | Bifunctional dihydrofolate reductase-thymidylate synthase | Pf3D7_04_v3: 748,088..749,914(+) | p.N51I (95/95) p.C59R (94/96) p.S108N (93/93) |
| MDR1 | PF3D7_0523000 | Multidrug resistance protein 1 | Pf3D7_05_v3: 957,890..962,149(+) | p.Y184F (39/89) p.D642G (6/86) p.N652D (39/84) |
| AAT1 | PF3D7_0629500 | Amino acid transporter AAT1 | Pf3D7_06_v3: 1,213,948..1,216,005(−) | p.S258L (80/96) |
| PPPK-DHPS | PF3D7_0810800 | Hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase | Pf3D7_08_v3: 548,200..550,616(+) | p.G437A (6/99) p.K540E (88/97) p.S436A/C(3/99) b |
| ACT | PF3D7_1036800 | “Acetyl-CoA transporter 1, putative” | Pf3D7_10_v3: 1,448,692..1,451,646(−) | p.T459I (76/91) p.I140V (8/94) |
| MRP2 | PF3D7_1229100 | Multidrug resistance-associated protein 2 | Pf3D7_12_v3: 1,192,888..1,199,214(−) |
p.L1531V (69,2/94) p.S1527T (74/94) p.S1371N (9/97) p.K714I (81/98) p.L199V (98/98) |
| CRN | PF3D7_1251200 | Coronin | Pf3D7_12_v3: 2,092,087..2,094,231(+) |
p.S183G (73/94) p.V424I (6/99) p.F434L (5/99) |
| K13 | PF3D7_1343700 | Kelch protein K13 | Pf3D7_13_v3: 1,724,817..1,726,997(−) |
p.K189T (10/91) |
| MDR2 | PF3D7_1447900 | Multidrug resistance protein 2 | Pf3D7_14_v3: 1,954,601..1,957,675(−) |
p.S635T (7/98) p.I492V (13/97) p.F423Y (19/99) p.S208N(79/93) |
a
Numbers of alternate alleles are shown in parentheses with total callable sites (of 120). Only alleles with an alternate reference found in at least five samples are shown with the exception of pppk-dhps.
b
Sensitive genotype: reference strain 3d7 is sulfadoxine resistant with an A437G mutation (33).