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. 2023 Oct 6;14(5):e01535-23. doi: 10.1128/mbio.01535-23

Fig 3.

Fig 3

Herp plays a key role in Mtb-mediated ROS production and intracellular survival of mycobacteria. (A) BMDMs were transfected with siControl or siHerp and infected with H37Ra (MOI = 5) for 0 and 24 h. Intracellular survival of H37Ra was measured using a CFU assay. (B) Herp-WT and Herp-KO Raw264.7 cells were infected with H37Ra (MOI = 5) for 0 and 24 h. The intracellular survival of H37Ra was measured using a CFU assay. (C) Herp-WT and Herp-KO Raw264.7 cells were infected with H37Ra (MOI = 5) for 24 h. Raw264.7 cells were treated with staurosporine (500 nM) for 6 h. Apoptosis was analyzed using Annexin-V/PI staining. Bar graphs depict the percentage of Annexin-V-positive cells. (D) BMDMs were transfected with siControl or siHerp and infected with H37Ra (MOI = 5) for 24 h. Levels of ROS were measured using dihydroethidium staining. Data are expressed as the mean ± standard error of the mean of three independent groups (A, B, and D). Data are expressed as the mean ± standard deviation of three independent experiments (C). N.S., not significant (P ≥ 0.05). *P < 0.05, **P < 0.01, and ***P < 0.001. STS, staurosporine; UN, untreated control.