Fig 1.

The effect of Rob1 on C. albicans filamentation is dependent upon strain background and inducing medium. (A) Homozygous ROB1 deletion mutants in the indicated strain backgrounds were spotted on agar plates prepared with Spider medium, RPMI medium, and RPMI supplemented with 10% bovine calf serum (BCS). The strains were incubated at 30°C or 37°C for 3–5 days prior to photographing. Representative images from three independent experiments are shown. (B) Overnight cultures of the indicated strains were diluted (1:50) into liquid RPMI + 10% BCS at 37°C. After 4 hours, the cultures were fixed and the distribution of yeast, pseudohyphae, and hyphae was determined by light microscopy. An asterisk indicates that the parental and rob1∆∆ mutant had statistically significant differences in distribution by Student’s t test (P < 0.05).