Fig 6.

The ROB1 alleles have distinct effects on the expression of canonical hyphae-associated genes during filamentation. The indicated strains were incubated in RPMI + 1% BCS for 4 hours and RNA isolated as described in the Materials and Methods. The expressions of ALS3, ECE1, HWP1, and ROB1 were determined by quantitative RT-PCR using the ∆∆CT method. The bars indicate mean of two independent experiments preformed in triplicate. Asterisks indicate statistically significant differences between the indicated strain and SN250 by ANOVA followed by Dunnett’s correction for multiple comparisons (P < 0.05).