Fig 2.
Survival under high-salt conditions as determined by LIVE/DEAD and CTC staining. A. baumannii ATCC 19606T was grown in minimal medium with 20 mM succinate and 300 mM NaCl to the stationary growth phase (timepoint 0 [T0]) and for further 7 days. Cells were stained with the LIVE/DEAD BacLight Bacterial Viability Kit according to manufacturer’s instructions and analyzed by flow cytometry. Representative density plots from at least three independent biological replicates are shown (A). The viable cell count (■) is defined as the sum of populations 6 and 7, while the total cell count (▲) is defined as the sum of populations 5–8 and quantified using the kit’s microsphere standard. The culturable cell count (●) was determined by serially diluting the culture and plating samples onto LB agar plates (B). The respiratory activity of cells either grown in low- (black bars) or high-salt (gray bars) mineral medium was determined by using the BacLight RedoxSensor CTC Vitality Kit according to manufacturer’s instructions. Cells were counterstained with DAPI and visualized with CLSM prior to counting (C). Error bars denote the standard deviation of the mean derived from at least three independent biological replicates.