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. 2023 Oct 5;14(5):e01655-23. doi: 10.1128/mbio.01655-23

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Copyright © 2023 Rolando et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Fig 3 — Ankyrin domains are essential for enzymatic activity of RomA. (A) Schematic representation of the RomA protein and the different deleted forms used in this study. (B) In vitro histone methyltransferase (HMTase) activity of RomA proteins using histone H3 as substrate. One microgram of recombinant GST-tagged proteins was incubated 1 hour at 30°C in the presence of 1 µg of histone H3 and 200 nCi of [¹⁴C]-SAM as substrate. GST-tagged proteins were detected with anti-GST antibodies and H3 loading with Coomassie Brillant Blue (CBB) staining.