Fig 6.

(A) AlphaFold2-based structural model of the BtsS dimer with bound pyruvate. Each BtsS monomer is represented as a green or light blue cartoon and the membrane boundaries are indicated by yellow lines. The position of a putative disulfide bridge between the monomers is encircled. (B) The putative pyruvate (Pyr) binding geometry (pyruvate in van der Waals representation) is shown enlarged in the right panel, including the arrangement of residues important for binding as stick model. (C) Schematic 2D contact view of pyruvate (Pyr) binding to BtsS. Hydrogen bonds between Arg72/99 and pyruvate as well as direct contacts to Cys110 are indicated. Ser113 contacts and stabilizes the Arg72 placement.