Fig 2.

PPP inhibition with CA reduces pyrin dephosphorylation and IL-1β release in BMDMs infected with ∆yopM Yptb. LPS-primed BMDMs were pretreated for 15 min and maintained with either DMSO or 10 nM CA and infected with the ∆yopM or ∆yopK strain at an MOI of 30 for 90 min. Treated uninfected BMDMs were analyzed in parallel. (A) Immunoblot analysis. Quantified immunoblot band intensity signals from one experiment representing PS205 pyrin/total pyrin are indicated below the respective blot image. (B) Mature IL-1β in supernatants as quantified by ELISA. Two-way ANOVA with Bonferroni post-hoc correction was applied to calculate the significance, and P-values of each treatment as compared to its corresponding DMSO-treated infected strain are indicated. P < 0.05 was considered significant; P < 0.001 (***); not significant (ns). Each data group is presented as an average (error bars are standard deviation) of at least three independent experiments.