Fig 6.

Longer pretreatment with 100 nM OA reduces pyrin dephosphorylation and IL-1β release in BMDMs infected with ∆yopM Yptb. LPS-primed BMDMs were pretreated for 3 h and maintained with either DMSO or 100 nM OA and infected with the ∆yopM Yptb at an MOI of 30 for 90 min. Treated uninfected BMDMs were analyzed in parallel. (A) Immunoblot analysis of BMDM lysates. Quantified immunoblot band intensity signals from one experiment representing PS205 pyrin/total pyrin are indicated below the respective blot image. (B) Mature IL-1β in supernatants as quantified by ELISA. Two-way ANOVA with Bonferroni post-hoc correction was applied to calculate the significance, and P-value of OA treatment as compared to its corresponding DMSO treated and ∆yopM infected sample is indicated. P < 0.05 was considered significant; P < 0.01 (**). Each data group is presented as an average (error bars are standard deviation) of at least three independent experiments.