Figure 3.

Gas chromatographic analyses of monoterpenol formation by recombinant MMR with menthone (B) and isomenthone (D) as substrates, and by recombinant MNR with menthone (C) and isomenthone (E) as substrates. The monoterpene standards (A) are menthone (1), isomenthone (2), neomenthol (3), neoisomenthol (4), menthol (5), and isomenthol (6). Assays for the conversion of isomenthone to monoterpenols (D and E) were scaled up 12-fold in time and 16-fold in protein concentration to increase product yield to that comparable for the conversions of menthone to monoterpenols (B and C).