FIG 1.

DENV4 and ZIKV promote phosphorylation of eIF2α in A549 cells. A549 cells were infected with DENV4 multiplicity of infection (MOI 2) or ZIKV (MOI 3) and harvested at the indicated times for analysis. (A) Immunoblot analysis of cell extracts resolved in denaturing SDS-PAGE. (B) Bar graph representative of flow cytometry analysis for quantification of cells expressing p-eIF2α within the total population (C) Dot plot and Venn diagram of representative results from co-staining with anti-flavivirus E protein and anti-p-eIF2α at 24 h.p.i. analyzed by flow cytometry and (D) immunofluorescence of infections under the same conditions, representative images of three independent experiments. In the column chart, the bars represent the means ± standard error of the mean from three independent experiments. Statistical analysis was performed by paired t test comparing infected samples to the respective uninfected control. **P ≤ 0.01, ***P ≤ 0.001. h.p.i., hours post-infection.