FIG 4.

DENV4, but not ZIKV, induces PKR-dependent translation arrest in infected cells. Immunofluorescence of A549 WT or PKR^−/− cells infected with DENV4 (A) or ZIKV (B) at 20 PFU of FFU/well for 48 h.p.i. in semi-solid medium. Cells labeled with puromycin before fixation and permeabilization. Co-staining with DAPI, anti-puromycin, anti-flavivirus envelope (E) and secondary antibodies. (C) Zoom on WT cells infected with ZIKV for a better appreciation of the immunostaining pattern. Representative images of three independent experiments. FFU, focus forming unit.