TABLE 1.
Comparison of SNPs across spontaneous mutants a
| Pos | Wt | rpoB-S450L | rpoB-H445Y | AA_POS | Effect | Locus_tag | Gene | Product |
|---|---|---|---|---|---|---|---|---|
| 555619 | T | T | C | 230/474 | Missense_variant c.688A > G p.Met230Val | Rv0465c | Probably transcriptional regulatory protein | |
| 761139 | C | C | T | 445/1172 | Missense_variant c.1333C > T p.His445Tyr | Rv0667 | rpoB | DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit) |
| 761155 | C | T | C | 450/1172 | Missense_variant c.1349C > T p.Ser450Leu | Rv0667 | rpoB | DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit) |
| 972980 | C | T | C | 243/386 | Missense_variant c.727G > A p.Gly243Ser | Rv0874c | Conserved hypothetical protein | |
| 1155197 | A | A | G | 492/709 | Missense_variant c.1474A > G p.Ile492Val | Rv1030 | kdpB | Probable potassium-transporting P-type ATPase B chain KdpB |
| 1319222 | C | C | T | 1330/1582 | Missense_variant c.3989C > T p.Ala1330Val | Rv1181 | pks4 | Probably polyketide beta-ketoacyl synthase Pks4 |
| 2300546 | A | T | A | 2147/4151 | Missense_variant c.6441T > A p.His2147Gln | Rv2048c | pks12 | Polyketide synthase Pks12 |
| 3690948 | C | C | T | |||||
| 3804941 | G | G | A | 26/236 | Missense_variant c.77G > A p.Cys26Tyr | Rv3390 | lpqD | Probable conserved lipoprotein LpqD |
| 3846791 | A | G | A | |||||
| 3846851 | A | G | A | |||||
| 3846852 | G | C | G | |||||
| 3846853 | C | T | C | |||||
| 3846857 | A | G | A | |||||
| 3846860 | G | T | G | |||||
| 3846866 | A | C | A |
a
Based on the whole-genome sequencing, all unique non-synonymous SNPs, including the intergenic ones, for the rpoB-S450L and rpoB-H445Y strains were determined in reference to the wt parental Mtb strain HN878. The only SNP shared by any of these strains and the previously characterized W_7642 is boldfaced and is present in the rpoB-H445Y strain.