Figure 4.

Protein-blot analysis and activity staining of OASTL and CAS contents. A, For the protein blot-analysis, total protein extracts were prepared, separated on SDS-gels, and blotted onto nitrocellulose membranes. Monospecific antibodies recognizing the OASTL and CAS proteins, respectively, were used for the immunoreaction. The Coomassie-stained gel loaded with the same samples is shown in the lowest section to demonstrate loading of equal protein amounts. B, After native PAGE with 150 μg protein/lane, the gel was subjected to activity staining.