TABLE 2.
Primer list for plasmid constructions
| Primer | Sequence |
|---|---|
| For overexpression plasmid construction | |
| HA-hOTUD6B -F(pCMV-HA) | 5′-gcccg aattc ggatgatatctaaggaaaagaaagctgcattg-3′ |
| HA-hOTUD6B -R(pCMV-HA) | 5′-ccg ctcgag cttagctgcaattttcagtaactatgtttacc-3′ |
| myc-hOTUD6B -F(pCMV-myc) | 5′-gccc gaattc ggatgatatctaaggaaaagaaagctgcattg-3′ |
| myc-hOTUD6B -R(pCMV-myc) | 5’-ccg ctcgag cagctgcaattttcagtaactatgtttacc-3’ |
| For point mutation plasmid construction | |
| OTUD6B CS-F | 5′-agattccatctgatggccac agt atgtataa-3′ |
| OTUD6B CS-R | 5′-tgtggccatcagatggaatctgtttaatttc-3′ |
| IRF3-K313R-F | 5′-atggcgaggtcccc agg gacaaggaa-3′ |
| IRF3-K313R-R | 5′-ctggggacctcgccatcaggcccatg-3′ |
| IRF3-K315R-F | 5′-aggtccccaaggac agg gaaggaggc-3′ |
| IRF3-K315R-R | 5′-ctgtccttggggacctcgccatcagg-3′ |
| IRF3-K360R-F | 5′-accagccgtggacc agg aggctcgtg-3′ |
| IRF3-K360R-R | 5′-ctggtccacggctggtcctggggcca-3′ |
| IRF3-K366R-F | 5′-ggctcgtgatggtc agg gttgtgccc-3′ |
| IRF3-K366R-R | 5′-ctgaccatcacgagcctcttggtcca-3′ |
| IRF3-K409R-F | 5′-cctccgaccagtac agg gcctacctg-3′ |
| IRF3-K409R-R | 5′-ctgtactggtcggaggtgagggagag-3′ |
| For truncation plasmid construction | |
| IRF3 ΔC-F | 5′- gccgaattcaatgggaaccccaaagccacg −3′ |
| IRF3 ΔC-R | 5′- gcctctagatcaccccggcaccaacagcc-3′ |
| IRF3 ΔN-F | 5′- gccgaattcagaagagtgggagttcgaggtgacag-3′ |
| IRF3 ΔN-R | 5′- gcctctagatcagctctccccagggccc-3′ |
| For shRNA knockdown plasmid construction | |
| OTUD6B Sh1-F(pLL3.7) | 5′-tgcaaagctactaacaggtgttttcaagagaaacacctgttagtagctttgcttttttc-3′ |
| OTUD6B Sh1-R(pLL3.7) | 5′-tcgagaaaaaagcaaagctactaacaggtgtttctcttgaaaacacctgttagtagctttgca-3′ |
| OTUD6B Sh2-F(pLL3.7) | 5′-tcgagaagaacggatagctgaattcaagagattcagctatccgttcttctcgttttttc-3′ |
| OTUD6B Sh2-R(pLL3.7) | 5′-tcgagaaaaaacgagaagaacggatagctgaatctcttgaattcagctatccgttcttctcg a-3′ |
| OTUD6B Sh3-F(pLL3.7) | 5′-tcgatgagactaatgcagtgaattcaagagattcactgcattagtctcatcgttttttc-3′ |
| OTUD6B Sh3-R(pLL3.7) | 5′-tcgagaaaaaacgatgagactaatgcagtgaatctcttgaattcactgcattagtctcatcga-3′ |