TABLE 2.
Effective stimulatory concentration and dissociation constants for newly characterized chemoeffectors
| Chemoreceptor | Chemoeffector | EC50 by FRET a (µM) | KD by ITC b (µM) |
|---|---|---|---|
| PA1608 (PctP) | Guanine | 0.6 ± 0.1 | |
| Guanosine | 0.4 ± 0.05 | ||
| Hypoxanthine | 1.6 ± 0.2 | KD1 = 43, KD2 = 286 | |
| Inosine | 8.2 ± 1.9 | ||
| Adenine | 39.5 ± 4.5 | ||
| Adenosine | 3.9 ± 0.8 | ||
| PA2654 (TlpQ) | 2-Phenylethylamine | 410 ± 12 | |
| Tyramine | 100 ± 7 | ||
| PA4307 (PctC) | 5-Aminovalerate | 1.5 ± 0.2 | 28 ± 2 |
| Methyl 4-aminobutyrate | 2.9 ± 0.4 | 129 ± 2 | |
| PA4309 (PctA) | L-Ornithine (L-Orn) | 1.8 ± 0.2 | 7.1 ± 0.5 |
| PA4310 (PctB) | L-Ornithine (L-Orn) | 10.2 ± 0.8 | 559 ± 80 |
| PA4915 | 2-Phenylethylamine | 58 ± 11 |
a
Half-maximal inhibitory ligand concentration (EC50) derived from dose-response curve measured by FRET.
b
Dissociation constants (KD ) obtained from ITC measurements.