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. 2023 Sep 15;14(5):e00611-23. doi: 10.1128/mbio.00611-23

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Copyright © 2023 Chakravarty et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Fig 6 — Increased AMPK activation in Tdrd7 knockout primary mouse cells. (A, B) A diagram showing the newly generated Tdrd7^fl/fl mice and the derived Tdrd7^Δ/Δ strain (A) and the genotypes of relevant strains (B). The location of primers (P) used for genotyping is shown in A. (C) Liver homogenates from Tdrd7^fl/fl and Tdrd7^Δ/Δ mice were analyzed for TDRD7 by immunoblot. (D) Tdrd7^fl/fl and Tdrd7^Δ/Δ primary MEFs were treated with IFNβ or polyI:C for 16 h, and the Tdrd7 mRNA levels were analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). (E) Tdrd7^fl/fl and Tdrd7^Δ/Δ primary MEFs were serum starved (SS) for the indicated times, and pAMPK was analyzed by immunoblot. (F) Tdrd7^fl/fl and Tdrd7^Δ/Δ primary splenocytes were serum starved (SS) and analyzed for pAMPK by immunoblot. The results are representative of three experiments using two to four mice from each group; the data represent mean ± SEM; *P < 0.05.