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Brrga1-d is resistant to GA-induced degradation. Immunoblot analysis using purified anti-RGA polyclonal antibodies on protein extracted from 6-d-old seedlings or 8-d-old seedlings pretreated with GA biosynthesis inhibitor, PAC, of Brrga1-d and wild-type B. rapa plants with (+) and without (−) GA treatment. Protein bands indicated with an arrow are BrRGA1 or Brrga1-d proteins, whereas those marked with an asterisk (*) represent nonspecific immunoreactive proteins.
