Figure 5.

Atrga-d is resistant to GA-induced degradation. Immunoblot results showing the levels of AtRGA or Atrga-d proteins in plants that were described in Figure 4A with (+) and without (−) GA application. Plants transformed with the wild-type transgene did not show accumulation of the wild-type repressor protein (data not shown). The GA-deficient mutant ga1-3 that accumulates higher amounts of AtRGA than wild type was used as a control. Protein bands indicated with arrows are AtRGA or Atrga-d proteins, whereas those marked with an asterisk (*) represent nonspecific immunoreactive proteins.