Fig 1.

Screening of pre-selected PGHs for high staphylolytic activity in murine serum. Twenty-eight highly active enzymes were expressed in a microwell plate format and challenged with 10⁷ CFU/mL S. aureus Newman in murine serum for 30 minutes. Each enzyme was scored based on the number of surviving bacteria (>20 colonies = 0; 10–20 colonies = 1; <10 colonies = 2; and 0 colonies = 3). Experiments were performed in technical and biological triplicates, resulting in a maximum possible score of 27 (dashed line). Stacked bars indicate the scores reached in individual biological replicates. As controls, pET21a_LST (+) and pET21a (horizontal gray bar) were used. PGHs are sorted according to their enzymatic activity profile, the domain structure is detailed in Table S2.