Figure 2.

(a) Karyograms from patient and isogenic LGMD2A lines indicating no genomic alterations occurrence during genome editing. (b) Immunocytochemistry for the pluripotency markers Oct4, Sox2, SSEA4, and TRA1-60 displaying the pluripotent state of the LGMD2A iPSC as well as their isogenic control iPSC after genome editing. Scale bars 100 μM. (c) Scatter plots comparing global gene expression profiles between H1 human ESC and LGMD2A iPSC, H9 human ESC and LGMD2A iPSC, CB iPSC, and LGMD2A iPSC, and H1 and H9 human ES cells (from left to right) and the corresponding Spearman correlation. H1 and H9 human ESC for analyses were acquired from the GSE73211 data set.