Figure 4.

McZTL is a clock-controlled gene in C₃ and CAM leaves of M. crystallinum. C₃- and CAM-induced M. crystallinum plants were entrained in LD (12/12) for 29 d (C₃) or 65 d (CAM). CAM-induced plants had been salt stressed for 19 d to ensure complete induction of CAM. At the start of the experiment, one-half of the plants were maintained in LD while one-half were transferred to LL. Leaf samples were collected at the indicated time points and RNA was isolated. Semiquantitative RT-PCR was performed on the RNA and the resulting band intensities were normalized to the McUBQ10 loading control. Diamonds, C₃ in LD; squares, C₃ in LL; triangles, CAM in LD; and circles, CAM in LL. A, McZTL transcript abundance. B, McFKF1 transcript abundance. C, McGI transcript abundance. D, McELF3 transcript abundance.