Figure 1.

Regulation of FER gene and FER protein expression by iron availability in roots. Wild-type and fer mutant plants were grown in the presence of 0.1, 10, or 100 μm FeNaEDTA. A, Semiquantitative RT-PCR analysis of FER mRNA levels in tomato roots. FER expression levels were normalized according to the constitutively expressed LeEF-1a gene. FER signals were absent in the fer plants due to the presence of an insertion within the region to be amplified. B, Western-blot analysis on total protein extracts; 9 μg protein were loaded in each lane. Coomassie Blue staining was used to demonstrate equal loading with proteins.