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. 2023 Oct 17;299(11):105354. doi: 10.1016/j.jbc.2023.105354

Figure 4.

Figure 4

O-GlcNAcylation enhances recruitment of PARG to DNA damage sites.A, 293T cells were transfected with Flag-PARG-WT or -S26A, together with HA-PCNA. B, cells were transfected with Flag-PARG and GFP-PARP1. C, 293T cells were transfected with Flag-PARG-WT, treated with TMG plus glucose and Etoposide (10 μM, 2 h). D–E, U2OS cells were transfected with mCherry-PARG, treated with laser micro-irradiation (arrows), and the mCherry signals quantitated. Scale bar, 10 μM. The laser microirradiation experiments were repeated three times, with 10 cells per experiment. Quantitation in (A–C) was done with Student’s t test, in E was done with a two-way ANOVA. ∗ indicates p < 0.05; ∗∗indicates p < 0.005. All western blots were repeated at least three times.