. 2023 Oct 4;89(10):e00331-23. doi: 10.1128/aem.00331-23

TABLE 4.

Summary of primers for Sanger sequencing

Sample ID	Primer type	Sequence (5′ to 3′)	Length (bp)	Tm (°C)^a	Gc (%)	Amplicon location (size)
GI_Set3^b	Forward primer	TCATTTTATGGTGATGATGAAAT	23	48.5	26.1	4889–5550 (662 bp)
GI_Set3^b	Reverse primer	AGGGGTCAATCATATTAACTTG	22	50.1	36.4	4889–5550 (662 bp)
GI_Set9^c	Forward primer	CCTTGCACATCTCAGGTGAATA	22	54.7	45.5	4703–5572 (870 bp)
GI_Set9^c	Reverse primer	TGAGGCCCTAACTGCAAATC	20	55.1	50.0	4703–5572 (870 bp)
GII_Set3^d	Forward primer	TTCTATGGTGATGATGAGATTGT	23	51.5	34.8	4612–5243 (632 bp)
GII_Set3^d	Reverse primer	CTAATCCAGGGGTCAATTACAT	22	52.0	40.9	4612–5243 (632 bp)
GII_Set9^e	Forward primer	CAATAGCACACTGGATCCTAAC	22	53.1	45.5	4459–5324 (866 bp)
GII_Set9^e	Reverse primer	CTAGCCAGATGTGCAAGATAAG	22	53.1	45.5	4459–5324 (866 bp)
GII_Set11^f	Forward primer	CCCATTCTCAAAGACCCATACA	22	54.5	45.5	4865–5693 (829 bp)
GII_Set11^f	Reverse primer	TGAGAACTCGGCACGAAAC	19	55.3	52.6	4865–5693 (829 bp)
GII_Set14^g	Forward primer	GATTTGAATGGTCTCACATTCTTG	24	52.4	37.5	4744–5336 (593 bp)
GII_Set14^g	Reverse primer	TTCTGGACCTAACTCTAAATCTAAC	25	51.8	36.0	4744–5336 (593 bp)

^{^a}

Melting temperatures were calculated with the default parameter sets of OligoAnalyzer (Integrated DNA Technology), which consider 0.25 μM of oligo concentration and 50 mM of Na⁺ concentration.

^{^b}

Reference sequence (Genbank ID: MT031988.1).

^{^c}

Reference sequence (Genbank ID: MW305499.1).

^{^d}

Reference sequence (Genbank ID: OP727614.1).

^{^e}

Reference sequence (Genbank ID: MW305576.1).

^{^f}

Reference sequence (Genbank ID: MZ478141.1).

^{^g}

Reference sequence (Genbank ID: OP686904.1).