TABLE 2.
Plasmids
| Plasmids | Characteristics | Source or reference |
|---|---|---|
| pUC19 | Apr, lacZ | (52)a |
| pMFY42 | Tcr, Kmr, RSF1010-based broad host range plasmid | (51)b |
| pYT11 | pUC19 derivative carrying mhpRABD (5.2-kb BglII-fragment) | (50)c |
| pMFYMhpRA | pUC19 derivative carrying mhpR and the promoter of mhp genes | This work |
| pMFYMhpScdY | pMFYMhpRA derivative carrying scdY | This work |
| pMFYMhpScdL1L2 | pMFYMhpRA derivative carrying scdL1L2 | This work |
| pMFYMhpScdL1L2Y | pMFYMhpRA derivative carrying scdL1L2Y | This work |
| pUCORF5(scdY)-Kmr | pUC19 derivative carrying DNA fragment containing scdK and scdY: :Kmr (SmaI site) | (44)d |
| pHSGtesBscdL1 | pHSG397 derivative carrying tesB and ScdL1 | This work |
| pUCScdM1M2F | pUC19 derivative carrying scdM1M2F | This work |
| pHSGScdL2NY-Kmr | pHSGtesBscdL1 derivative with scdK, scdY: :Kmr, and scdM1M2F | This work |
pSuperCosI* (Stratagene, CA, USA).
Vieira, J., and Messing, J. 1987. Methods Enzymol. 153: 3–11.
Nagata Y. et al. 1993. J Bacteriol 175:6403–6410.
Arai H. et al. 1999. Microbiology 145:2813–2820.
Horinouchi M, et al. 2018. Appl Environ Microbiol 84:e01324-18.
Kmr: Km resistance.
pSuperCosI* (Stratagene, CA).