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. 2023 Oct 10;89(10):e01050-23. doi: 10.1128/aem.01050-23

TABLE 2.

Plasmids

Plasmids Characteristics Source or reference
pUC19 Apr, lacZ (52)a
pMFY42 Tcr, Kmr, RSF1010-based broad host range plasmid (51)b
pYT11 pUC19 derivative carrying mhpRABD (5.2-kb BglII-fragment) (50)c
pMFYMhpRA pUC19 derivative carrying mhpR and the promoter of mhp genes This work
pMFYMhpScdY pMFYMhpRA derivative carrying scdY This work
pMFYMhpScdL1L2 pMFYMhpRA derivative carrying scdL1L2 This work
pMFYMhpScdL1L2Y pMFYMhpRA derivative carrying scdL1L2Y This work
pUCORF5(scdY)-Kmr pUC19 derivative carrying DNA fragment containing scdK and scdY: :Kmr (SmaI site) (44)d
pHSGtesBscdL1 pHSG397 derivative carrying tesB and ScdL1 This work
pUCScdM1M2F pUC19 derivative carrying scdM1M2F This work
pHSGScdL2NY-Kmr pHSGtesBscdL1 derivative with scdK, scdY: :Kmr, and scdM1M2F This work

pSuperCosI* (Stratagene, CA, USA).

a

Vieira, J., and Messing, J. 1987. Methods Enzymol. 153: 3–11.

b

Nagata Y. et al. 1993. J Bacteriol 175:6403–6410.

c

Arai H. et al. 1999. Microbiology 145:2813–2820.

d

Horinouchi M, et al. 2018. Appl Environ Microbiol 84:e01324-18.

e

Kmr: Km resistance.

f

pSuperCosI* (Stratagene, CA).