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. 2023 Sep 8;8(5):e00141-23. doi: 10.1128/msystems.00141-23

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Copyright © 2023 Calderon Toledo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Fig 3 — Bacterial adherence and cytotoxicity to human epithelial cells. (a)Differences in the number of bacteria attached to Caco-2 monolayers according to the source of isolation (environmental and clinical) or (b)ETEC expressing CS23 versus ETEC isolates expressing other CFs (non-CS23) of each group. C, clinical and E, environmental. Assays were performed in triplicate, and the violin plots show the distribution of the data and the mean and SD. The asterisks indicate a significant difference in the unpaired t-test (*P < 0.05 and **P < 0.01) using GraphPad Prism 8.00. (c)Cytotoxicity to Caco-2 cells infected by environmental and clinical ETEC isolates.