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Relative activities of the recombinant ZOG1 and cisZOG1 enzymes with selected cytokinins
Recombinant ZOG1 and cisZOG1 were incubated with 500 μm cytokinin and 1 mm UDPG. The relative activity is presented as the percentage of zeatin (for ZOG1) or cis-zeatin (for cisZOG1).
| Substrate
|
Relative Activity (%)
|
|
|---|---|---|
| ZOG1 | cisZOG1 | |
| trans-Zeatin | 100 | 14 |
| cis-Zeatin | 3 | 100 |
| o-Topolin | 0.1 | 490 |
| m-Topolin | 23 | 0 |
| p-Topolin | 0.1 | 0 |
| TDZ | 0 | 0 |
| (o-OH)TDZ | 0 | 128 |
| (m-OH)TDZ | 0 | 0 |
| (p-OH)TDZ | 0 | 0 |
