Table II.
Inhibitors used in this studya
Symbolb | Inhibitor/Reagentc | Target/Effectd | Infiltrate XXO | Spray O3 |
---|---|---|---|---|
μme | μmf | |||
Van | Metavanadate | ATPases | 50,000 | 100,000 |
Lan | LaCl3 | Ca2+ channels | 1,000 | 2,000 |
Ama | α-Amanitin | Transcription | 2.2 | 4.4 |
Hba | Herbimycin A | Tyr kinases | 1,000 | 2,000 |
K25 | K252a | Ser/Thr kinases | 2.2 | 4.4 |
Pep | Pepstatin | Asp proteases | 1.0 | |
E-64 | E-64 | Cys proteases | 1.0 | |
VAD | z-VAD-fmk | Caspases | 50 | |
PMSF | PMSF | Ser proteases | 500 | |
Ion | A23187 | Ca2+ ionophore | 10 | |
Ca | CaCl2 | Increased Ca2+ | 2,000 | |
Mg | MgCl2 | Control | 2,000 | |
EGTA | EGTA | Decreased Ca2+ | 1,000 | |
Gd | Gd(III)Cl3 | Ca2+ channels | 1,000 |
Inhibitors used in this study with their respective abbreviations, concentrations used, and proposed targets.
Abbreviations used in the figures for each inhibitor.
Full name of all inhibitors and reagents used.
Proposed inhibitor target or the expected effect of the treatments.
Concentrations used for in vitro coinfiltration experiments with XXO as the radical source.
Concentrations used for pretreating plants by spraying intact plants with the inhibitor 1 h prior to O3 exposure.