FIG 1.

Ffar4 knockout significantly impaired glucose tolerance at ZT12-14. CRISPR–Cas9 gene editing strategy for total Ffar4 KO mice. Genomic sequencing identified the deletion of 16- and 7-bp nucleotides from the first exon of Ffar4 (a). Day and night food intake (WT/KO mice, n = 6) (b), body weight per week (WT/KO mice, n = 10) (c) between WT and KO mice were recorded. Blood glucose at each time point in GTT (d), AUCs at each ZT (e) between WT and KO mice were performed using two-way ANOVA (WT/KO mice, n = 13 per ZT). Data are expressed as the mean ± standard error of the mean. P < 0.05 was considered statistically significant using two-way ANOVA. * represents P < 0.05；** represents P < 0.01；*** represents P < 0.001；**** represents P < 0.0001, ANOVA, analysis of variance; KO, knockout; NS, not significant; WT, wild type.