Figure S1. Schematic presentation of experiments.

Pregnant outbred Swiss mice were treated from E6.5 to E15.5, with thia doses of 0.6, and 6 mg/kg/day, and control mice “0” received only vehicle (oil). F1 and F3 mice were euthanized at embryonic day 15, postnatal day 35, and at the age of 2 mo. Schema of breeding described in “Mouse treatment and dissection” section of Materials and Methods. For spermatozoa DNA methylation analysis, F2 mice were also euthanized.