Table I.
Differentially expressed genes validated by qRT-PCR
The values listed in the four columns on the right are the log2(hypoxia/normoxia) values measured by microarrays and qRT-PCR for wild-type (WT) and transgenic PSAG12:ipt (T5) plants.
| AGI Locus
|
Common Name
|
Forward Primer (5′→3′)
|
Reverse Primer (5′→3′)
|
Microarray
|
Real-Time PCR
|
||
|---|---|---|---|---|---|---|---|
| WT | T5 | WT | T5 | ||||
| At1g77120 | Alcohol dehydrogenase | GAATCGCTGGTGCTTCTAGG | CTCAGCGATCACCTGTTGAA | 3.88 | 3.88 | 3.13 | 2.82 |
| At3g43190 | Suc synthase, putative | ACAAACTCAACGGGCAATTC | AAAGAGCAGGCTGCACAAAT | 4.08 | 3.78 | 3.30 | 2.93 |
| At5g20830 | Suc synthase | CGCCGTTACCTTGAAATGTT | CTTCAAACACCGGAACCACT | 3.19 | 3.24 | 2.03 | 1.99 |
| At4g33070 | Pyruvate decarboxylase 1 (PDC1) | GGTGGTCCTAAGTTGCGTGT | CTGCTCCCCAATAAGTTCCA | 4.33 | 4.48 | 3.59 | 3.65 |
| At4g17260 | Lactate dehydrogenase (LDH1) | TGGTGGTGATGTTTTCCTCA | CTGCAGCTTCTCAGCCTCTT | 1.80 | 2.09 | 0.10 | 1.55 |
| At2g31390 | Putative fructokinase | TCCTTTGTCGGTGCTCTTCT | CTGAGGGAAGAGCTGGAATG | 1.87 | 1.57 | 1.18 | 0.90 |
| At4g26520 | Fru-bisphosphate aldolase | GCACAGTCCCACCAGCTATT | CCGCCAAAAGAGAAAGTGAG | 0.89 | 0.76 | 0.54 | −0.58 |
| At4g32840 | Phosphofructokinase family protein | CGATCTCCCCACTTATCCAA | GGCCTGCACGTCTAAAATGT | 2.02 | 1.38 | 1.18 | 0.77 |
| At2g36580 | Putative pyruvate kinase | CTGTTGTCATTCCCCGACTT | CATGGGGAAAAGACCTCTGA | 1.11 | 1.03 | 0.83 | 0.76 |
| At1g72330 | Putative Ala aminotransferase | AGAGCCGAAGGAGCAATGTA | TTGCAGTAGAACGCATCTGG | 0.68 | −0.03 | 0.00 | −0.14 |
| At5g51460 | Trehalose-6-P phosphatase (AtTPPA) | CCGATGATCGATAAGGTGCT | TGTGCAACCAATGTCCAGTT | 1.04 | 1.16 | 1.08 | 0.91 |
| At5g65140 | Trehalose-6-P phosphatase, putative | TTCGGTCGGTGGTAAAGAAC | TCAAGAGCCTTGCCTTTGTT | 1.95 | 1.87 | 1.17 | 1.41 |
| At4g24040 | Trehalase-like protein | AAACCGTGTTTTGGAACGAG | TAGACGCAAAGACGTTGGTG | 1.37 | 0.85 | 0.65 | 0.66 |
| At1g76640 | Putative calmodulin | GGCTGTGTTTGCTTACATGG | CCTCGGCTTCTTCATCAGAC | 1.78 | 1.52 | 0.81 | −0.46 |
| At1g76650 | Putative calmodulin | CATGGATGCAAACAGAGACG | ACAGCAGCTACGGCTTCTTC | 2.87 | 2.65 | 3.41 | 3.36 |
| At2g19590 | ACC oxidase | CTCAGCAAGACGATGGATGA | TTGGACCAGAAAAGGCATTC | 2.76 | 2.68 | 1.88 | 1.85 |
| At3g23150 | Ethylene receptor ETR2 | TTGTGCTACTGCGATTACGC | CAACTTCACGACCAAGCTCA | 1.10 | 0.60 | 0.42 | −0.15 |
| At3g11930 | Ethylene-responsive protein, putative | GTGGAGAAGCAGCCAGAGAC | CCATTGGAGAGCGTAGAAGC | 1.90 | 1.48 | 0.84 | 0.86 |
| At2g34390 | Major intrinsic family protein (NIP2;1) | TTACTGTCTCGGCCACCTCT | CCAAGGTTGATCCGATGACT | 4.55 | 4.31 | 4.66 | 3.88 |
| At5g60660 | Major intrinsic family protein (PIP2;4) | CCGATGGCTACAACAAAGGT | CACGTGAGAGTCACGAGCAT | −1.21 | −1.57 | −1.52 | −1.25 |
| At5g47450 | Major intrinsic family protein (TIP2;3) | GCAGCTGATCCAAAGAAAGG | ACCTTGCTGGATTCATGGAG | −2.32 | −2.43 | −2.99 | −2.18 |
| At3g02550 | Lateral organ boundary domain protein (LBD41) | AAGCTTGTGGGAGGATTGTG | TGACCGGTTCTCCTTTCATC | 3.10 | 2.90 | 1.51 | 1.70 |
| At2g14210 | MADS-box protein (ANR1) | GAGAGATGGGGAGAGGGAAG | TTCTGCATCACAAAGGATCG | 3.04 | 2.40 | 2.87 | 2.68 |
| At2g16060 | Nonsymbiotic hemoglobin protein (GLB1) | AACACTTTGAGGTGGCCAAG | CAGCTTTAATGGCAGCAACA | 3.37 | 3.19 | 2.53 | 2.61 |
| At2g17850 | Putative senescence-associated rhodanese protein | CCAAACCGATCATCTCATCC | TGACCCAAGCAATGTAACCA | 4.28 | 4.58 | 3.81 | 3.83 |
| At2g39510 | Nodulin MtN21 family protein | GCCTTCAATCCATTGAGCAT | TTGCTTTTTCCCCACAAAAC | 3.56 | 3.69 | 2.22 | 2.07 |
| At2g47460 | Putative MYB family transcription factor | TGATGGGGAGTTGCATAACA | ACTCCACCGATGGACAAGAC | 1.07 | 1.20 | 0.60 | 0.48 |
| At2g47520 | Putative AP2 domain transcription factor | GCCAAACTGAATTTCCCAAA | CATCAGGTCCTCCGATAAGC | 3.18 | 2.83 | 2.61 | 2.63 |
| At5g54470 | CONSTANS B-box zinc-finger family protein | CTGAATCTTGGCCCAACTGT | GCTCCGTCGTTACAACCAAT | 1.75 | 1.29 | 1.42 | 1.43 |