TABLE 1.
Primers used for qPCR
| Gene | Primer sequence (5′−3′) |
|---|---|
| E-cadherin | Forward: 5′- ACCAGCAGTTCGTTGTCGTCAC-3′ |
| Reverse: 5′- GTTCCTCGTTCTCCACTCTCACATG-3′ | |
| ZO-1 | Forward: 5′- AACCCGAAACTGATGCTGTGGATAG −3' |
| Reverse: 5′- CGCCCTTGGAATGTATGTGGAGAG −3′ | |
| ZO-2 | Forward: 5′- CATGTCTCTAACGGATGCTCGGAAG −3′ |
| Reverse: 5′- GTTTAGGGCTGGGATGTTGATGAGG −3′ | |
| Occludin | Forward: 5′- TGGAGGCTATGGCTATGGCTATGG-3′ |
| Reverse: 5′-TTACTAAGGAAGCGATGAAGCAGAAGG-3′ | |
| ZEB | Forward: 5′- AAGCCATACGAATGCCCGAACTG-3′ |
| Reverse: 5′- GCGAGGAACACTGAGATGTCTTGAG-3′ | |
| NF-κB | Forward: 5′-CCATAGCCATAGTTGCGGTCCTTC-3′ |
| Reverse: 5′- CGTTCTTCCCTCCCTTTTCCTTTCC-3′ | |
| IKB-α | Forward:5′-GAATCACCAGAACATCGTGAAG-3′ |
| Reverse: 5′- CAGTACTCCATGATTAGCACCT-3′ | |
| TNF-α | Forward:5′- CTCA TTCCTGCTTGTGGC −3′ |
| Reverse: 5′- CACTTGGTGGTTTGCTACG −3′ | |
| IL-1β | Forward: 5′- TTCCCA TTAGACAACTGC-3′ |
| Reverse: 5′- CTGTAGTGTTGTA TGTGA TC −3′ | |
| IL-6 | Forward: 5′- CAGAACCGCAGTGAAGAG −3′ |
| Reverse: 5′- CAGAACCGCAGTGAAGAG −3′ | |
| IL-10 | Forward: 5′- CAGAGCCAAAGCAGTGAGC −3′ |
| Reverse: 5′- TGACCCAGTCCATCCAGAG −3′ | |
| GRP78 | Forward: 5′-GTCAGGGAGAGGAGGAAT-3′ |
| Reverse: 5′-TGGTGTCACTTATGGTAGAA-3′ | |
| IRE1 | Forward:5′-TTGAAGTGGACAGTGAAGG-3′ |
| Reverse: 5′-TTGAAGTGGACAGTGAAGG-3′ | |
| PERK | Forward: 5′-GTAGCCACGACCTTCATC-3′ |
| Reverse: 5′-GTAGCCACGACCTTCATC-3′ | |
| ATF6 | Forward: 5′-TGCCTTGGGAGTCAGACCTATGG-3′ |
| Reverse: 5′-CTGTGGACCGAGGAGAGGAGATG-3′ | |
| EIF2α | Forward: 5′-TGGTGGTTATCCGTGTTG-3′ |
| Reverse: 5′-CCGATTGCTTGAAGATGTC-3′ | |
| xBP1 | Forward: 5′-TTGGGCATTCTGGACAAGTTGGAC-3′ |
| Reverse: 5′-AAAGGGAGGCTGGTAAGGAACTAGG-3′ | |
| ATF4 | Forward: 5′-TCTGCCTTCTCCAGGTGGTTCC-3′ |
| Reverse: 5′-GCTGCTGTCTTGTTTTGCTCCATC-3′ | |
| CHOP | Forward: 5′-CTACTCTTGACCCTGCGTCCCTAG-3′ |
| Reverse: 5′-TCTTCCTTGCTCTTCCTCCTCTTCC-3′ | |
| Caspase8 | Forward: 5′-CATCCTGACTGGCGTGAACTATGAC-3′ |
| Reverse: 5′-GTGAAGGTGGGCTGTGGCATC-3′ | |
| RIPK1 | Forward: 5′-CGACTTCCAGACACCAAGCCATC-3′ |
| Reverse: 5′-TTTCCACTGCCTTCCCAGGTTTTC-3′ | |
| RIPK3 | Forward: 5′-GAAGACACGGCACTCCTTGGTATC-3′ |
| Reverse: 5′-CTTGAGGCAGTAGTTCTTGGTGGTG-3′ | |
| MLKL | Forward: 5′-ACAGGCTACACCATTCGGAAACAC-3′ |
| Reverse: 5′-TCTGCTTTAGTGCTCTTTGCTGTCC-3′ | |
| Bax | Forward: 5′-GCTACAGGGTTTCATCCAGGATCG-3′ |
| Reverse: 5′-TGCTGTCCAGTTCATCTCCAATTCG-3′ | |
| Bcl-2 | Forward: 5′-CCGTCGTGACTTCGCAGAGATG-3′ |
| Reverse: 5′-ATCCCTGAAGAGTTCCTCCACCAC-3′ | |
| CytC | Forward: 5′-AGGTGCCCGACTTCTCTGACTATC-3′ |
| Reverse: 5′-CCGCATAAGCAACACCCACAGTAG-3′ | |
| Caspase12 | Forward: 5′-CCGTCCAGAGCACCAGTCCTC-3′ |
| Reverse: 5′-GCTTCACCCCACAGATTCCTTCC-3′ | |
| Caspase9 | Forward: 5′-GCTCCAAGGACGACTTCATCAAGG-3′ |
| Reverse: 5′-CGCACTGCTCAGCTCACACTC-3′ | |
| Caspase3 | Forward: 5′-ATGCTGCTCCCTTCCTCTTCCTC-3′ |
| Reverse: 5′-CACTTGTGTCTGTTGTTGCTGCTG-3′ | |
| MLC | Forward:5′-GATAGCCATCAGCAGCCTCACATC-3′ |
| Reverse: 5′- GCAACAGGAGCAGCAGGAGAAC-3′ | |
| RhoA | Forward:5′-ACGGTGTTTGAAAACTATGTGG-3′ |
| Reverse: 5′- GACAGAAATGCTTGACTTCTGG-3′ | |
| ROCK | Forward: 5′-GGGTGGTAGACTGGAGGGTTGG-3′ |
| Reverse: 5′-GGTAGGGTTTCTTCTGGGCTTTCTG-3′ | |
| CaM | Forward:5′-ACAAGGATGGGAATGGTTACAT-3′ |
| Reverse: 5′- TGCAGTCATCATCTGTACGAAT-3′ | |
| MLCK | Forward:5′-GGGCTGCCTCTCATCATCAATACG-3′ |
| Reverse: 5′- TGGATTCTGCTTCTGTGGGTAGGG-3′ | |
| β-actin | Forward: 5′-AATCCTGCGGCATCCACGAAAC-3′ |
| Reverse: 5′-CAGCACCGTGTTGGCGTAGAG-3′ |