TABLE 2.
Antibodies required for western blot
| Name | Cat no. | Company | Dilution times |
|---|---|---|---|
| β-actin | AC026 | ABclonal Technology | 1:1,000 |
| GADPH | D16H11 | Cell Signaling Technology | 1:1,000 |
| ZEB | 70,512T | Cell Signaling Technology | 1:1,000 |
| E-cadherin | 60335-1-Ig | Proteintech | 1:2,000 |
| ZO-1 | 13,663T | Cell Signaling Technology | 1:1,000 |
| GRP78 | 3,177S | Cell Signaling Technology | 1:1,000 |
| ATF6 | WL01153 | WanLei Bio | 1:500 |
| PERK | 20582-1-AP | Proteintech | 1:1,000 |
| IRE1 | 27528-1-AP | Proteintech | 1:1,000 |
| CHOP | 15204-1-AP | Proteintech | 1:1,000 |
| Caspase8 | WL03426 | WanLei Bio | 1:1,500 |
| p-RIPK1 | 53,286T | Cell Signaling Technology | 1:1,000 |
| p-RIPK3 | 91,702T | Cell Signaling Technology | 1:1,000 |
| p-MLKL | 37,333T | Cell Signaling Technology | 1:1,000 |
| Bax | WL01637 | WanLei Bio | 1:500 |
| Bcl-2 | WL01556 | WanLei Bio | 1:500 |
| Caspase12 | WL03268 | WanLei Bio | 1:1,000 |
| Caspase9 | WL01551 | WanLei Bio | 1:500 |
| Caspase3 | WL04004 | WanLei Bio | 1:500 |
| p-MLC | AF8618 | Affinity | 1:500 |
| RhoA | 10749-1-AP | Proteintech | 1:1,000 |
| ROCK | 21850-1-AP | Proteintech | 1:1,000 |