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Purification of xanthan lyase
| Step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Crude enzyme | 3,384 | 452 | 0.134 | 100 | 1.0 |
| DEAE-cellulose | 44.0 | 99.2 | 2.25 | 21.9 | 16.8 |
| Ammonium sulfate (50–90%) | 16.0 | 44.6 | 2.79 | 9.87 | 20.8 |
| Sephacryl S-200HR | 5.98 | 20.3 | 3.39 | 4.49 | 25.3 |
| Butyl-Toyopearl 650M | 2.12 | 7.44 | 3.51 | 1.65 | 26.2 |
| QAE-Sephadex A-25 | 0.61 | 3.83 | 6.28 | 0.847 | 46.9 |
