TABLE 2.
Effect of various compounds on xanthan lyase activity
| Compound | Concn (mM) | Activitya (%) |
|---|---|---|
| None (control) | 100 | |
| CaCl2 | 1 | 94.0 |
| CoCl2 | 1 | 144 |
| CuCl2 | 1 | 96.8 |
| HgCl2 | 1 | 2.80 |
| MgCl2 | 1 | 88.0 |
| MnCl2 | 1 | 87.1 |
| ZnCl2 | 1 | 93.1 |
| NaCl | 150 | 29.5 |
| KCl | 150 | NDb |
| Dithiothreitol | 1 | 95.4 |
| Glutathione (reduced form) | 1 | 98.6 |
| 2-Mercaptoethanol | 1 | 125 |
| N-Ethylmaleimide | 1 | 67.3 |
| Iodoacetamide | 1 | 28.1 |
| EDTA | 1 | 68.7 |
a
Reactions was carried out for 10 min at 30°C, pH 5.5 (sodium acetate buffer), in the presence or absence (control) of the compound listed. The activity of the control was taken as 100%.
b
ND, not detected.