. 2023 Nov 16;13(11):e10727. doi: 10.1002/ece3.10727

TABLE 2.

Primer sequence, amplicon size, genomic coordinates (assemblies Sp. aurata: GCA_900880675.2; D. labrax: GCA_000689215.1) and annealing temperature for each studied genomic region.

Species	Gene	Genomic coordinates	Annealing temperature (°C)	Primer sequence (5′‐3′)	Amplicon size (bp)
Dicentrarchus labrax	vgll3	9,203,305–9,203,951 ^a	63	TACCCTCCCCGATACCTGG	646
		9,203,305–9,203,951 ^a	63	TGTGTGGACAGTGCAGGAC	646
		9,202,574–9,203,170	61	TCTCTCCCTGTCTCCTCCTC	596
		9,202,574–9,203,170	61	CTGGGATGGATAGGTGCTGT	596
		9,201,705–9,202,720	63	TGCCTGGATGTGATGTACCA	1015
		9,201,705–9,202,720	63	CTGGTGTCGCAGGTCCCT	1015
		9,201,381–9,201,802	61	GGAGCGCTCTGAAAACTTGT	421
		9,201,381–9,201,802	61	TGTTGTTGCTGGTGATGGTG	421
	six6	11,589,507–11,590,303	61	TCTTTCTTTAACACCGCCCG	796
		11,589,507–11,590,303	61	ACACCGACTCGTTCTTGTTG	796
		11,590,632–11,591,488 ^a	63	GGCTACAGGACTTACACCCA	856
		11,590,632–11,591,488 ^a	63	AAGTACCACAGCAAGATCGC	856
		11,588,596–11,589,739	61	GCTATCTATCCGCCCTCTTATTT	1149
		11,588,596–11,589,739	61	CCCTCCGATGATTTCCTATTGG	1149
		11,592,056–11,592,572	61	GAATGTGACATCTGACGGGC	515
		11,592,056–11,592,572	61	CGAAGAGACAGAGCAGATCG	515
Sparus aurata	vgll3	24,911,798–24,912,208 ^a	63	AACGTCTATCACCCTCACCC	410
		24,911,798–24,912,208 ^a	63	ACCAAACTGACGTCTTTGCT	410
		24,910,950–24,911,675	63	GCTCCCATTCTGCTCCCA	725
		24,910,950–24,911,675	63	CCGGGTGGTTGAAGGAGAC	725
		24,910,316–24,911,343	63	GAGCGCACTACCTCCCTG	1027
		24,910,316–24,911,343	63	CACCACGTCGCCAATGTC	1027
		24,909,934–24,910,621	61	CCAATCACAAGCGCTCTGAA	687
		24,909,934–24,910,621	61	CCGACTTTTAGCGCATGGTT	687
	six6	14,404,868–14,405,650	61	GTCTTTCTTTAACAGCGTGCG	782
		14,404,868–14,405,650	61	GCACCGACTCGTTCTTGTTG	782
		14,406,022–14,406,799 ^a	63	AACCGCAGACAAAGAGACAG	777
		14,406,022–14,406,799 ^a	63	ACCCCTTATTAAACAACAAGCAC	777
		14,403,964–14,405,087	63	GTGTGTCTGATACTAAAGCCGA	1123
		14,403,964–14,405,087	63	TCTATTGGACTTGGCAGATTGG	1123
		14,407,370–14,407,840	63	GTCATAGTGCCTGCGTTGAG	470
		14,407,370–14,407,840	63	CGAAGAGACAGAGCAGATCG	470

^{^a}

Containing polymorphic SNPs.