TABLE 2.
Results of PCR amplifications with the different sets of primers for nirKa
| Species or strainb | Source or referenceb | Denitrificationc | nirKc | PCR productsd determined with primers:
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| nirK1F-nirK3R | nirK1F-nirK4R | nirK1F-nirK5R | nirK2F-nirK3R | nirK2F-nirK4R | nirK2F-nirK5R | ||||
| Alcaligenes xylosoxidans NCIMB 11015 | NCIMB | + | + | + | + | + | − | − | − |
| Hyphomicrobium zavarzinii IFAM ZV-622T (ATCC 27496) | ATCC | + | + | + | − | + | − | − | − |
| Ochrobactrum anthropi LMG 2136 | LMG | + | + | + | + | + | + | + | + |
| Rhizobium meliloti Rm1021 | C. Elmerich, Institut Pasteur, Paris, France | + | + | + | − | + | + | − | + |
| Rhizobium meliloti 20115 | C. Elmerich, Institut Pasteur, Paris, France | + | + | + | − | + | + | − | + |
| Rhodobacter sphaeroides f. sp. denitrificans | Satoh et al. (20) | + | + | + | − | + | +e | − | + |
| Alcaligenes sp. strain (DSM 30128) | DSM | + | NDg | + | + | + | +e | − | + |
| Alcaligenes xylosoxidans subsp. denitrificans DSM 30026 | DSM | + | ND | + | + | + | − | − | − |
| Blastobacter denitrificans IFAM 1005 (DSM 1113) | DSM | + | ND | + | − | + | − | − | + |
| Enterobacter agglomerans 339 | W. Klingmüller, Universität Bayreuth, Bayreuth, Germany | − | − | −f | − | − | −f | −f | −f |
| Enterobacter cloacae NCIMB 11463 | NCIMB | − | − | − | − | − | −f | − | − |
| Escherichia coli K-12 (DSM 498) | DSM | − | − | −f | − | − | −f | −f | −f |
| Klebsiella pneumoniae subsp. pneumoniae DSM 681 | DSM | − | − | −f | − | − | −f | − | − |
a
Touchdown reaction from 45 to 40°C used.
b
ATCC, American Type Culture Collection; DSM, Deutsche Sammlung von Mikroorganismen; IFAM, Institut für Allgemeine Mikrobiologie, Universität Kiel, Kiel, Germany. LMG, Laboratorium voor Mikrobiologie, Universiteit Gent, Ghent, Belgium; NCIMB, National Collections of Industrial and Marine Bacteria, Aberdeen, United Kingdom.
c
Data from literature.
d
+, PCR product of the expected size; −, no PCR product.
e
Expected PCR products and weaker products of unexpected and nonspecific size.
f
No expected PCR products, only products of unexpected and nonspecific size.
g
ND, not determined.