TABLE 3.
Results of PCR amplifications with the different sets of primers for nirSa
| Strainb | Source or referenceb | Denitrificationc | nirSc | PCR productsd determined with primers:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| nirS1F-nirS3R | nirS1F-nirS5R | nirS1F-nirS6R | nirS2F-nirS3R | nirS2F-nirS5R | nirS2F-nirS6R | nirS3F-nirS5R | nirS3F-nirS6R | nirS4F-nirS5R | nirS4F-nirS6R | ||||
| Alcaligenes eutropus H16 (DSM 428) | DSM | + | + | + | + | + | + | − | + | − | − | − | − |
| Alcaligenes faecalis A15 | H. Bothe, Universität Köln, Cologne, Germany | + | + | + | − | + | + | − | + | + | + | − | + |
| Azospirillum brasilense Sp7 (DSM 1690) | DSM | + | + | − | − | + | − | − | + | − | + | − | + |
| Paracoccus denitrificans ATCC 19367 | ATCC | + | + | + | − | + | + | + | + | + | − | + | + |
| Pseudomonas aeruginosa DSM 6195 | DSM | + | + | − | − | +e | − | + | − | − | − | − | + |
| Pseudomonas stutzeri ATCC 14405 | ATCC | + | + | + | + | + | + | + | + | + | + | + | + |
| Roseobacter denitrificans ATCC 33942T | ATCC | + | + | + | + | + | + | + | +e | + | + | − | − |
| Alcaligenes eutrophus DSM 530 | DSM | + | NDg | + | + | + | + | − | + | − | − | − | − |
| Denitrifying isolate IFAM 3698 | IFAM | + | ND | − | − | + | + | + | +e | − | + | − | + |
| Escherichia coli K-12 (DSM 498) | DSM | − | − | − | −f | − | −f | −f | −f | −f | −f | − | − |
| Enterobacter agglomerans 339 | W. Klingmüller, Universität Bayreuth, Bayreuth, Germany | − | − | − | − | − | − | −f | −f | − | −f | − | − |
| Enterobacter cloacae NCIMB 11463 | NCIMB | − | − | − | − | − | − | − | −f | −f | − | − | − |
| Klebsiella pneumoniae subsp. pneumoniae DSM 681 | DSM | − | − | − | − | − | − | − | − | − | − | − | − |
a
Touchdown reaction from 45 to 40°C used.
b
See Table 2, footnote b.
c
Data from literature.
d
+, PCR product of the expected size; −, no PCR product.
e
Expected PCR products and weaker products of unexpected and nonspecific size.
f
No expected PCR products, only products of unexpected and nonspecific size.
g
ND, not determined.