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. 2023 Nov 16;14:7419. doi: 10.1038/s41467-023-43288-x

Fig. 2. Meiotic midbody remnant formation.

Fig. 2

a Representative confocal images of MKLP1 (magenta) and cell boundaries (actin, green) at Metaphase II. On the left, whole egg image with membrane delineated with dotted, white circle is shown. Square indicates the region shown in the zoom on the right side with XY, YZ, and XZ views with a three-dimensional coordinate system with axes depicting orientation of the different views. Asterisk marks the polar body. b Representative still images from confocal live-cell imaging of oocytes undergoing cytokinesis, expressing MKLP1-mCherry (magenta) and GAP43-GFP (gray). Stages of meiosis are labeled. Timepoint 00:00 represents the beginning of Anaphase I onset. Below is a zoom of the mMBR at Metaphase II and a cartoon interpretation. c Representative confocal images showing localization of CHMP4B (magenta) relative to microtubules (green; tubulin) and to the MB region in early Telophase I (top panels), Late Telophase I (middle panels), and Metaphase II (bottom panels). Arrowheads indicate bands of CHMP4B enrichment. All experiments were conducted three times. d Schematic summarizing the timing of formation mMB cap, its regression and mMBR formation.