Fig. 5. Genome-wide disconnect between HSF1 binding and transcriptional activation.

a The numbers of observed HSF1 sequence motifs and ChIP-seq peaks in the vicinity of promoter regions (blue) compared with motifs and peaks in these regions expected at random (white). Observed motifs are based on homer’s findmotif output for hg19 genome +/−1 kb from the TSSs. Expected motifs are calculated assuming even distribution of the same number of HSF1 sequence motifs (~ 286 K) and HSF1 peaks (~ 18 K) across the genome. b Fractions of activated promoters in HS (blue) and As (red) in MCF7 (dark blue, dark red) and K562 (light blue, light red) cells. Approximately 50% (in HS) and 20% (in As) of commonly activated genes showed HSF1 at their promoters. c Fractions of HSF1 peaks at all gene promoters in HS (blue) and As (red) in MCF7 (dark blue, dark red) and K562 (light blue, light red) cells, at promoters common to both or exclusive to each cell line. d The expected and observed numbers of HSF1 sequence motifs and ChIP-seq peaks are shown around dTREs as in (a). Unlike promoters, dTREs are cell line specific, so that HSF1 sequence motifs are shown separately for each cell line. e HS-activated dTREs between K562 and MCF7 cells. Venn diagram showing their overlap between the two cell lines. The percentages of dTREs with HSF1 peaks for dTREs common between the cell lines and exclusive dTREs for each cell line are shown underneath. f Same as in (e) except all dTREs are shown.