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. 2023 Oct 19;31:100737. doi: 10.1016/j.omto.2023.100737

Figure 1.

Figure 1

uORF of RNF10 encoded a peptide in mice CT26 cells

(A) A volcano plot, showing the green, red, and black points represent significantly downregulated (green) or upregulated (red) RING finger (RNF)-protein-encoding genes in CT26 cells and normal colon tissue; black represents genes with no significant expression change. x axis: log2 ratio of RNA expression levels between normal and tumor tissues. y axis: the false discovery rate value (−log10 transformed) of RNF-protein-encoding genes. The blue dot indicates RNF10. (B) An anti-FLAG tag antibody was used to detect RNF10 uPeptide expression in HEK293T cells transfected with the above vectors. (C) Total protein, from HEK293T cells transfected with p-uORF or the control plasmid, was separated via SDS-PAGE. Individual gel bands (10–15 kDa) were extracted and subjected to liquid chromatography with tandem mass spectrometry (LC-MS/MS); the RNF10 uPeptide-specific peptide was identified (lower). (D) RNF10 uPeptide expression was detected in CT26 cells or normal colon tissue of BALB/c mice.