Figure 4.

Induction of efficient tumor control in a CT26 colon cancer mouse model immunized with a long RNF10 uPeptide vaccine

(A) Tumor growth (mean ± SEM) in untreated (control) or RNF10-uPeptide immunized BALB/C mice (n = 7), inoculated subcutaneously (s.c.) with CT26 cells. (B) Upper panel, CD8-stained cancer tissue sections. Scale bar, 20 μm. Lower panel, proportional CD8⁺ lymphocyte areas in tumor tissue of control (n = 5) or vaccinated (n = 5) animals. (C) Tumor-infiltrating lymphocytes (TILs) or splenocytes were stimulated with PMA and ionomycin to assess the ability of CD8⁺ T cells to produce IFN-γ and TNF-α. Cytokine production was determined by intracellular cytokine staining and flow cytometry. Data are representative of two independent experiments. Percentages of dead tumor cells are shown (mean ± SD, n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, compared with untreated T cells (−). p-values were determined by two-tailed one-way ANOVA with Dunnett’s multiple-comparisons test.