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. 2023 Oct 19;31:100737. doi: 10.1016/j.omto.2023.100737

Figure 5.

Figure 5

The RNF10 uPeptide is expressed in pancreatic cancer

(A) HEK293T cells were transfected with the p-uORF expression vector carrying an expression cassette for human RNF10 uORF with a 3×FLAG-coding sequence. Immunoblotting for FLAG expression using an anti-FLAG tag antibody in the indicated cells (n = 3). (B) Liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis, following SDS-PAGE, was performed to identify epitopes within the human RNF10 uPeptide. (C) Expression (measured and normalized as transcripts per million base pairs [TPM] from the RNA-seq data) of RNF10 in pancreatic carcinoma patient samples from TCGA. The statistical significance of differences was evaluated using the Student’s t test: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (D) Six randomly selected pancreatic carcinoma patient samples and their paired adjacent normal pancreatic tissues were collected. The expression of human RNF10 uPeptide was detected by immunoblotting.