Figure 2.

Western blotting analysis

(A) Western blot analysis showing protein levels of dystrophin-glycoprotein complex composing proteins, including α-DG, β-DG, α-actin, desmin, cTnT, and cardiac cTnI, in day 48 WT, Δ3–7, and Δ3–9 hiPSC-CMs. GAPDH was used as a loading control. (B) Quantification of protein expression in (A). (C) Western blot analysis showing protein levels of dystrophin-associated cardioprotective proteins, including AHNAK, Cypher, and CRYAB, in day 48 WT, Δ3–7, and Δ3–9 hiPSC-CMs. GAPDH was used as a loading control. (D) Quantification of protein expression in (C). (E) Western blot analysis of α-actin, desmin, TnT, AHNAK, Cypher, and CRYAB in WT and Δ3–9 hiPSC-CM lysates (input) and dystrophin IP samples. (F) Signal intensity ratio of α-actin, desmin, TnT, AHNAK, Cypher, and CRYAB to Dp427m in dystrophin IP samples in (E). Data are presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005.