T287 phosphorylation and oxidation of CaMKII; phosphorylation of PLB is elevated in Δ3–7 hiPSC-CMs
(A) Western blot analysis showing that protein levels of phosphorylated CaMKII and oxidized CaMKII were elevated in D3–7 hiPSC-CMs. GAPDH was used as a loading control. (B) Quantification of protein expression of CaMKIIδ, phosphorylated (T287) and oxidized (M281/M282) CaMKII, and phosphorylated PLB (T17). Data are presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005.